rabbit anti pmarcks Search Results


donkey anti-rabbit igg alexa 488  (Thermo Fisher)
90
Thermo Fisher donkey anti-rabbit igg alexa 488
Donkey Anti Rabbit Igg Alexa 488, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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phosphorylated marcks  (Cell Signaling Technology Inc)
94
Cell Signaling Technology Inc phosphorylated marcks
A Accumulation of MAPK, PI3K/AKT, YAP and PKC signalling effectors including total and <t>phosphorylated</t> DUSP6, <t>MARCKS,</t> ERK, YAP, S6 and AKT 24 h after treatment with vehicle control (−) or 10 nM Trametinib (+). Western blot analyses were performed using three independent biological replicates ( n = 3). kD kilodalton. REVERT total protein loading stain is shown in Supplementary Fig. . B Expression levels of pS6 and DUSP6 (normalised to total S6 or REVERT staining, respectively) in vehicle control or 10 nM Trametinib-treated GNAQ/GNA11 -mutant (solid dot) or wild-type (crossed dot) UM cell lines. Data derived from three independent biological experiments ( n = 3), and P values were calculated using paired t tests. ns not significant. C Percentage of cells undergoing S-phase inhibition (dotted line set at 50% S-phase inhibition) and change in % sub-G1 (dotted line set at 30% sub-G1) in GNAQ/GNA11 -mutant (solid circle) or wild-type (crossed circle) UM cell lines treated with 5 µM IDE196 (data derived from ), 10 nM Trametinib or 2 µM BEZ235. Data derived from three independent biological experiments ( n = 3).
Phosphorylated Marcks, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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anti pmarcks ser167 170  (Cell Signaling Technology Inc)
93
Cell Signaling Technology Inc anti pmarcks ser167 170
A Accumulation of MAPK, PI3K/AKT, YAP and PKC signalling effectors including total and <t>phosphorylated</t> DUSP6, <t>MARCKS,</t> ERK, YAP, S6 and AKT 24 h after treatment with vehicle control (−) or 10 nM Trametinib (+). Western blot analyses were performed using three independent biological replicates ( n = 3). kD kilodalton. REVERT total protein loading stain is shown in Supplementary Fig. . B Expression levels of pS6 and DUSP6 (normalised to total S6 or REVERT staining, respectively) in vehicle control or 10 nM Trametinib-treated GNAQ/GNA11 -mutant (solid dot) or wild-type (crossed dot) UM cell lines. Data derived from three independent biological experiments ( n = 3), and P values were calculated using paired t tests. ns not significant. C Percentage of cells undergoing S-phase inhibition (dotted line set at 50% S-phase inhibition) and change in % sub-G1 (dotted line set at 30% sub-G1) in GNAQ/GNA11 -mutant (solid circle) or wild-type (crossed circle) UM cell lines treated with 5 µM IDE196 (data derived from ), 10 nM Trametinib or 2 µM BEZ235. Data derived from three independent biological experiments ( n = 3).
Anti Pmarcks Ser167 170, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 93 stars, based on 1 article reviews
anti pmarcks ser167 170 - by Bioz Stars, 2026-03
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rabbit anti-pmarcks (ser152/156  (Millipore)
90
Millipore rabbit anti-pmarcks (ser152/156
A Accumulation of MAPK, PI3K/AKT, YAP and PKC signalling effectors including total and <t>phosphorylated</t> DUSP6, <t>MARCKS,</t> ERK, YAP, S6 and AKT 24 h after treatment with vehicle control (−) or 10 nM Trametinib (+). Western blot analyses were performed using three independent biological replicates ( n = 3). kD kilodalton. REVERT total protein loading stain is shown in Supplementary Fig. . B Expression levels of pS6 and DUSP6 (normalised to total S6 or REVERT staining, respectively) in vehicle control or 10 nM Trametinib-treated GNAQ/GNA11 -mutant (solid dot) or wild-type (crossed dot) UM cell lines. Data derived from three independent biological experiments ( n = 3), and P values were calculated using paired t tests. ns not significant. C Percentage of cells undergoing S-phase inhibition (dotted line set at 50% S-phase inhibition) and change in % sub-G1 (dotted line set at 30% sub-G1) in GNAQ/GNA11 -mutant (solid circle) or wild-type (crossed circle) UM cell lines treated with 5 µM IDE196 (data derived from ), 10 nM Trametinib or 2 µM BEZ235. Data derived from three independent biological experiments ( n = 3).
Rabbit Anti Pmarcks (Ser152/156, supplied by Millipore, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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pmarcks antibody  (Cell Signaling Technology Inc)
90
Cell Signaling Technology Inc pmarcks antibody
A Accumulation of MAPK, PI3K/AKT, YAP and PKC signalling effectors including total and <t>phosphorylated</t> DUSP6, <t>MARCKS,</t> ERK, YAP, S6 and AKT 24 h after treatment with vehicle control (−) or 10 nM Trametinib (+). Western blot analyses were performed using three independent biological replicates ( n = 3). kD kilodalton. REVERT total protein loading stain is shown in Supplementary Fig. . B Expression levels of pS6 and DUSP6 (normalised to total S6 or REVERT staining, respectively) in vehicle control or 10 nM Trametinib-treated GNAQ/GNA11 -mutant (solid dot) or wild-type (crossed dot) UM cell lines. Data derived from three independent biological experiments ( n = 3), and P values were calculated using paired t tests. ns not significant. C Percentage of cells undergoing S-phase inhibition (dotted line set at 50% S-phase inhibition) and change in % sub-G1 (dotted line set at 30% sub-G1) in GNAQ/GNA11 -mutant (solid circle) or wild-type (crossed circle) UM cell lines treated with 5 µM IDE196 (data derived from ), 10 nM Trametinib or 2 µM BEZ235. Data derived from three independent biological experiments ( n = 3).
Pmarcks Antibody, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/pmarcks antibody/product/Cell Signaling Technology Inc
Average 90 stars, based on 1 article reviews
pmarcks antibody - by Bioz Stars, 2026-03
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pmarcks  (Cell Signaling Technology Inc)
94
Cell Signaling Technology Inc pmarcks
A Accumulation of MAPK, PI3K/AKT, YAP and PKC signalling effectors including total and <t>phosphorylated</t> DUSP6, <t>MARCKS,</t> ERK, YAP, S6 and AKT 24 h after treatment with vehicle control (−) or 10 nM Trametinib (+). Western blot analyses were performed using three independent biological replicates ( n = 3). kD kilodalton. REVERT total protein loading stain is shown in Supplementary Fig. . B Expression levels of pS6 and DUSP6 (normalised to total S6 or REVERT staining, respectively) in vehicle control or 10 nM Trametinib-treated GNAQ/GNA11 -mutant (solid dot) or wild-type (crossed dot) UM cell lines. Data derived from three independent biological experiments ( n = 3), and P values were calculated using paired t tests. ns not significant. C Percentage of cells undergoing S-phase inhibition (dotted line set at 50% S-phase inhibition) and change in % sub-G1 (dotted line set at 30% sub-G1) in GNAQ/GNA11 -mutant (solid circle) or wild-type (crossed circle) UM cell lines treated with 5 µM IDE196 (data derived from ), 10 nM Trametinib or 2 µM BEZ235. Data derived from three independent biological experiments ( n = 3).
Pmarcks, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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rabbit anti-flag  (Millipore)
90
Millipore rabbit anti-flag
A Accumulation of MAPK, PI3K/AKT, YAP and PKC signalling effectors including total and <t>phosphorylated</t> DUSP6, <t>MARCKS,</t> ERK, YAP, S6 and AKT 24 h after treatment with vehicle control (−) or 10 nM Trametinib (+). Western blot analyses were performed using three independent biological replicates ( n = 3). kD kilodalton. REVERT total protein loading stain is shown in Supplementary Fig. . B Expression levels of pS6 and DUSP6 (normalised to total S6 or REVERT staining, respectively) in vehicle control or 10 nM Trametinib-treated GNAQ/GNA11 -mutant (solid dot) or wild-type (crossed dot) UM cell lines. Data derived from three independent biological experiments ( n = 3), and P values were calculated using paired t tests. ns not significant. C Percentage of cells undergoing S-phase inhibition (dotted line set at 50% S-phase inhibition) and change in % sub-G1 (dotted line set at 30% sub-G1) in GNAQ/GNA11 -mutant (solid circle) or wild-type (crossed circle) UM cell lines treated with 5 µM IDE196 (data derived from ), 10 nM Trametinib or 2 µM BEZ235. Data derived from three independent biological experiments ( n = 3).
Rabbit Anti Flag, supplied by Millipore, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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rabbit anti phospho myosin light chain mlc  (Cell Signaling Technology Inc)
96
Cell Signaling Technology Inc rabbit anti phospho myosin light chain mlc
A Accumulation of MAPK, PI3K/AKT, YAP and PKC signalling effectors including total and <t>phosphorylated</t> DUSP6, <t>MARCKS,</t> ERK, YAP, S6 and AKT 24 h after treatment with vehicle control (−) or 10 nM Trametinib (+). Western blot analyses were performed using three independent biological replicates ( n = 3). kD kilodalton. REVERT total protein loading stain is shown in Supplementary Fig. . B Expression levels of pS6 and DUSP6 (normalised to total S6 or REVERT staining, respectively) in vehicle control or 10 nM Trametinib-treated GNAQ/GNA11 -mutant (solid dot) or wild-type (crossed dot) UM cell lines. Data derived from three independent biological experiments ( n = 3), and P values were calculated using paired t tests. ns not significant. C Percentage of cells undergoing S-phase inhibition (dotted line set at 50% S-phase inhibition) and change in % sub-G1 (dotted line set at 30% sub-G1) in GNAQ/GNA11 -mutant (solid circle) or wild-type (crossed circle) UM cell lines treated with 5 µM IDE196 (data derived from ), 10 nM Trametinib or 2 µM BEZ235. Data derived from three independent biological experiments ( n = 3).
Rabbit Anti Phospho Myosin Light Chain Mlc, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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rabbit anti total mlc  (Cell Signaling Technology Inc)
97
Cell Signaling Technology Inc rabbit anti total mlc
A Accumulation of MAPK, PI3K/AKT, YAP and PKC signalling effectors including total and <t>phosphorylated</t> DUSP6, <t>MARCKS,</t> ERK, YAP, S6 and AKT 24 h after treatment with vehicle control (−) or 10 nM Trametinib (+). Western blot analyses were performed using three independent biological replicates ( n = 3). kD kilodalton. REVERT total protein loading stain is shown in Supplementary Fig. . B Expression levels of pS6 and DUSP6 (normalised to total S6 or REVERT staining, respectively) in vehicle control or 10 nM Trametinib-treated GNAQ/GNA11 -mutant (solid dot) or wild-type (crossed dot) UM cell lines. Data derived from three independent biological experiments ( n = 3), and P values were calculated using paired t tests. ns not significant. C Percentage of cells undergoing S-phase inhibition (dotted line set at 50% S-phase inhibition) and change in % sub-G1 (dotted line set at 30% sub-G1) in GNAQ/GNA11 -mutant (solid circle) or wild-type (crossed circle) UM cell lines treated with 5 µM IDE196 (data derived from ), 10 nM Trametinib or 2 µM BEZ235. Data derived from three independent biological experiments ( n = 3).
Rabbit Anti Total Mlc, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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rabbit anti-dscam  (Millipore)
90
Millipore rabbit anti-dscam
A Accumulation of MAPK, PI3K/AKT, YAP and PKC signalling effectors including total and <t>phosphorylated</t> DUSP6, <t>MARCKS,</t> ERK, YAP, S6 and AKT 24 h after treatment with vehicle control (−) or 10 nM Trametinib (+). Western blot analyses were performed using three independent biological replicates ( n = 3). kD kilodalton. REVERT total protein loading stain is shown in Supplementary Fig. . B Expression levels of pS6 and DUSP6 (normalised to total S6 or REVERT staining, respectively) in vehicle control or 10 nM Trametinib-treated GNAQ/GNA11 -mutant (solid dot) or wild-type (crossed dot) UM cell lines. Data derived from three independent biological experiments ( n = 3), and P values were calculated using paired t tests. ns not significant. C Percentage of cells undergoing S-phase inhibition (dotted line set at 50% S-phase inhibition) and change in % sub-G1 (dotted line set at 30% sub-G1) in GNAQ/GNA11 -mutant (solid circle) or wild-type (crossed circle) UM cell lines treated with 5 µM IDE196 (data derived from ), 10 nM Trametinib or 2 µM BEZ235. Data derived from three independent biological experiments ( n = 3).
Rabbit Anti Dscam, supplied by Millipore, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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rabbit anti ps6  (Cell Signaling Technology Inc)
98
Cell Signaling Technology Inc rabbit anti ps6
A Accumulation of MAPK, PI3K/AKT, YAP and PKC signalling effectors including total and <t>phosphorylated</t> DUSP6, <t>MARCKS,</t> ERK, YAP, S6 and AKT 24 h after treatment with vehicle control (−) or 10 nM Trametinib (+). Western blot analyses were performed using three independent biological replicates ( n = 3). kD kilodalton. REVERT total protein loading stain is shown in Supplementary Fig. . B Expression levels of pS6 and DUSP6 (normalised to total S6 or REVERT staining, respectively) in vehicle control or 10 nM Trametinib-treated GNAQ/GNA11 -mutant (solid dot) or wild-type (crossed dot) UM cell lines. Data derived from three independent biological experiments ( n = 3), and P values were calculated using paired t tests. ns not significant. C Percentage of cells undergoing S-phase inhibition (dotted line set at 50% S-phase inhibition) and change in % sub-G1 (dotted line set at 30% sub-G1) in GNAQ/GNA11 -mutant (solid circle) or wild-type (crossed circle) UM cell lines treated with 5 µM IDE196 (data derived from ), 10 nM Trametinib or 2 µM BEZ235. Data derived from three independent biological experiments ( n = 3).
Rabbit Anti Ps6, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 98/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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rabbit polyclonal anti receptor  (Cell Signaling Technology Inc)
98
Cell Signaling Technology Inc rabbit polyclonal anti receptor
A Accumulation of MAPK, PI3K/AKT, YAP and PKC signalling effectors including total and <t>phosphorylated</t> DUSP6, <t>MARCKS,</t> ERK, YAP, S6 and AKT 24 h after treatment with vehicle control (−) or 10 nM Trametinib (+). Western blot analyses were performed using three independent biological replicates ( n = 3). kD kilodalton. REVERT total protein loading stain is shown in Supplementary Fig. . B Expression levels of pS6 and DUSP6 (normalised to total S6 or REVERT staining, respectively) in vehicle control or 10 nM Trametinib-treated GNAQ/GNA11 -mutant (solid dot) or wild-type (crossed dot) UM cell lines. Data derived from three independent biological experiments ( n = 3), and P values were calculated using paired t tests. ns not significant. C Percentage of cells undergoing S-phase inhibition (dotted line set at 50% S-phase inhibition) and change in % sub-G1 (dotted line set at 30% sub-G1) in GNAQ/GNA11 -mutant (solid circle) or wild-type (crossed circle) UM cell lines treated with 5 µM IDE196 (data derived from ), 10 nM Trametinib or 2 µM BEZ235. Data derived from three independent biological experiments ( n = 3).
Rabbit Polyclonal Anti Receptor, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 98/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Image Search Results


A Accumulation of MAPK, PI3K/AKT, YAP and PKC signalling effectors including total and phosphorylated DUSP6, MARCKS, ERK, YAP, S6 and AKT 24 h after treatment with vehicle control (−) or 10 nM Trametinib (+). Western blot analyses were performed using three independent biological replicates ( n = 3). kD kilodalton. REVERT total protein loading stain is shown in Supplementary Fig. . B Expression levels of pS6 and DUSP6 (normalised to total S6 or REVERT staining, respectively) in vehicle control or 10 nM Trametinib-treated GNAQ/GNA11 -mutant (solid dot) or wild-type (crossed dot) UM cell lines. Data derived from three independent biological experiments ( n = 3), and P values were calculated using paired t tests. ns not significant. C Percentage of cells undergoing S-phase inhibition (dotted line set at 50% S-phase inhibition) and change in % sub-G1 (dotted line set at 30% sub-G1) in GNAQ/GNA11 -mutant (solid circle) or wild-type (crossed circle) UM cell lines treated with 5 µM IDE196 (data derived from ), 10 nM Trametinib or 2 µM BEZ235. Data derived from three independent biological experiments ( n = 3).

Journal: Oncogenesis

Article Title: PKC-independent PI3K signalling diminishes PKC inhibitor sensitivity in uveal melanoma

doi: 10.1038/s41389-024-00511-8

Figure Lengend Snippet: A Accumulation of MAPK, PI3K/AKT, YAP and PKC signalling effectors including total and phosphorylated DUSP6, MARCKS, ERK, YAP, S6 and AKT 24 h after treatment with vehicle control (−) or 10 nM Trametinib (+). Western blot analyses were performed using three independent biological replicates ( n = 3). kD kilodalton. REVERT total protein loading stain is shown in Supplementary Fig. . B Expression levels of pS6 and DUSP6 (normalised to total S6 or REVERT staining, respectively) in vehicle control or 10 nM Trametinib-treated GNAQ/GNA11 -mutant (solid dot) or wild-type (crossed dot) UM cell lines. Data derived from three independent biological experiments ( n = 3), and P values were calculated using paired t tests. ns not significant. C Percentage of cells undergoing S-phase inhibition (dotted line set at 50% S-phase inhibition) and change in % sub-G1 (dotted line set at 30% sub-G1) in GNAQ/GNA11 -mutant (solid circle) or wild-type (crossed circle) UM cell lines treated with 5 µM IDE196 (data derived from ), 10 nM Trametinib or 2 µM BEZ235. Data derived from three independent biological experiments ( n = 3).

Article Snippet: Membranes were incubated at 4 °C overnight in primary antibodies diluted in Intercept Blocking Buffer (TBS) (Li-Cor, Lincoln, NE, USA) or Odyssey Blocking Buffer (Li-Cor) with Tween 20 (0.05%), as follows: total MARCKS (1:1000, 2C2, WH0004082M6, Sigma-Aldrich), phosphorylated MARCKS (pMARCKS; Ser 152/156 , 1:1000, 2741 S, Cell Signaling Technology, Danvers, MA, USA), DUSP6 (1:250 or 1:1000, EPR129Y, ab76310, Abcam, Cambridge, UK), total AKT (1:1000, 40D4, 2920S, Cell Signaling Technology), phosphorylated AKT (pAKT; Ser 473 , 1:100, D9E, 4060S, Cell Signaling Technology), pAKT (Ser 473 , 1:500, 736E11, 3787, Cell Signaling Technology), total ribosomal S6 (1:500, 54D2, 2317 S, Cell Signaling Technology), phosphorylated ribosomal S6 (pS6; Ser 235/236 , 1:1000, 2F9, 4856S, Cell Signaling Technology), total YAP (1:500, 1A12, 12395S, Cell Signaling Technology), phosphorylated YAP (pYAP; Ser 127 , 1:2000, 4911, Cell Signaling Technology), total ERK (1:2 000, 137F5, 4695S, Cell Signaling Technology), phosphorylated ERK (pERK; Tyr 204 , 1:250, E-4, SC-7383, Santa Cruz, Dallas, TX, USA), and MEK1/2 (1:500, L38C12, 4694S, Cell Signaling Technology).

Techniques: Control, Western Blot, Staining, Expressing, Mutagenesis, Derivative Assay, Inhibition

A Fold change in DUSP6 and pS6 expression (normalised log 2 protein expression in drug-treated – normalised log 2 protein expression in control-treated cells) in GNAQ/GNA11 -mutant (solid circle) and wild-type (crossed circle) UM cell lines treated with 5 µM IDE196, 10 nM Trametinib, or 2 µM BEZ235. Data compared using one-way ANOVA with the Geisser–Greenhouse correction and Tukey’s multiple comparison test, adjusted P values are shown. Data derived from three independent biological experiments ( n = 3, mean ± SD). B Accumulation of MAPK, PI3K, YAP and PKC signalling effectors, including total and phosphorylated DUSP6, MARCKS, ERK, YAP, S6 and AKT 24 h after treatment with vehicle control (−) or 2 µM BEZ235 (+). Western blot analyses were performed using three independent biological replicates ( n = 3). kD kilodalton. REVERT total protein loading stain is shown in Supplementary Fig. .

Journal: Oncogenesis

Article Title: PKC-independent PI3K signalling diminishes PKC inhibitor sensitivity in uveal melanoma

doi: 10.1038/s41389-024-00511-8

Figure Lengend Snippet: A Fold change in DUSP6 and pS6 expression (normalised log 2 protein expression in drug-treated – normalised log 2 protein expression in control-treated cells) in GNAQ/GNA11 -mutant (solid circle) and wild-type (crossed circle) UM cell lines treated with 5 µM IDE196, 10 nM Trametinib, or 2 µM BEZ235. Data compared using one-way ANOVA with the Geisser–Greenhouse correction and Tukey’s multiple comparison test, adjusted P values are shown. Data derived from three independent biological experiments ( n = 3, mean ± SD). B Accumulation of MAPK, PI3K, YAP and PKC signalling effectors, including total and phosphorylated DUSP6, MARCKS, ERK, YAP, S6 and AKT 24 h after treatment with vehicle control (−) or 2 µM BEZ235 (+). Western blot analyses were performed using three independent biological replicates ( n = 3). kD kilodalton. REVERT total protein loading stain is shown in Supplementary Fig. .

Article Snippet: Membranes were incubated at 4 °C overnight in primary antibodies diluted in Intercept Blocking Buffer (TBS) (Li-Cor, Lincoln, NE, USA) or Odyssey Blocking Buffer (Li-Cor) with Tween 20 (0.05%), as follows: total MARCKS (1:1000, 2C2, WH0004082M6, Sigma-Aldrich), phosphorylated MARCKS (pMARCKS; Ser 152/156 , 1:1000, 2741 S, Cell Signaling Technology, Danvers, MA, USA), DUSP6 (1:250 or 1:1000, EPR129Y, ab76310, Abcam, Cambridge, UK), total AKT (1:1000, 40D4, 2920S, Cell Signaling Technology), phosphorylated AKT (pAKT; Ser 473 , 1:100, D9E, 4060S, Cell Signaling Technology), pAKT (Ser 473 , 1:500, 736E11, 3787, Cell Signaling Technology), total ribosomal S6 (1:500, 54D2, 2317 S, Cell Signaling Technology), phosphorylated ribosomal S6 (pS6; Ser 235/236 , 1:1000, 2F9, 4856S, Cell Signaling Technology), total YAP (1:500, 1A12, 12395S, Cell Signaling Technology), phosphorylated YAP (pYAP; Ser 127 , 1:2000, 4911, Cell Signaling Technology), total ERK (1:2 000, 137F5, 4695S, Cell Signaling Technology), phosphorylated ERK (pERK; Tyr 204 , 1:250, E-4, SC-7383, Santa Cruz, Dallas, TX, USA), and MEK1/2 (1:500, L38C12, 4694S, Cell Signaling Technology).

Techniques: Expressing, Control, Mutagenesis, Comparison, Derivative Assay, Western Blot, Staining